![]() ![]() Īs reported in other studies and our own experiences in a rat model for abdominal aortic aneurysm, oxygen supply prevents hypoxia during the surgical procedure. In rats, arterial blood samples can be obtained by catheter placement in the tail artery. Moreover, acid-base status of anaesthetized animals can be monitored by analyses of arterial blood samples, which is valuable during long periods of anaesthesia and when normal physiological parameters are important for the outcome of the study. For this reason, pulsoximetry should always be performed during surgical procedures in rodents for monitoring vital signs during anaesthesia. Opiates cause respiratory depression, which can be seen by a reduced respiratory rate and low blood oxygen saturation. ![]() Other drug combinations may also cause hypoxia, such as combinations containing the opioids fentanyl or sufentanil. Xylazine has a detrimental effect on oxygenation of the peripheral blood, as it induced pulmonary oedema with a rapid onset, cellular damage and pleural fluid, in combination with a transient hypertension, followed by hypotension, bradycardia and heart block. ![]() As a reason for mortality, drug induced hypoxia was suggested. In rats anaesthetized by ketamine and xylazine, oxygen supply during the procedure reduced mortality from 58% to 17% during bile duct ligation. It is well known that oxygen supply during surgery in rodents improves post-operative recovery. We concluded that oxygen supply improved oxygen saturation and avoided hypoxia but did not have an influence on the acid-base status. All rats developed respiratory acidosis, which could not be reversed by supplemental oxygen supply. We found that oxygen supply after surgery prevented hypoxia but did not result in a significant difference in the blood acid-base status. The peripheral blood oxygen saturation and arterial acid-base status were analyzed for differences between the two groups. Twelve Sprague Dawley rats underwent surgery and were divided in two groups, breathing either 100% oxygen or atmospheric air during a post-operative period. Hypoxia may be extended during recovery, and for this reason we wished to investigate if oxygen supply during a one hour post-operative period reduced the development of hypoxia and respiratory acidosis in rats anaesthetized with fentanyl/fluanisone and midazolam. In anaesthetic practice the risk of hypoxia and arterial blood gas disturbances is evident, as most anaesthetic regimens depress the respiratory function. ![]()
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